Our Unique Services

Gale-Onn labs puts in great efforts in successfully applying and developing groundbreaking methods and technics all for the purpose achieving pregnancy even for couples who experience repeated failures in past treatments. Such methods and technics include , inter alia, the following:

• 1. Ideal preparation of the sperm prior to injection to the uterus (IUI: Intra Unit Insemination);
• 2. Thoroughly analyzing past failure of IUI or IVF (In Vitro Fertilization) treatment;
• 3. Thoroughly analyzing cause for apparent lack of sperm cells seen in standard sperm test (Azoospermia).

A. Ideal preparation of the sperm prior to injection to the uterus – while there are many reasons for pregnancy failure, as for the sperm the two main reasons are: weak movability which prevents it from reaching the egg and abnormalities in the sperm cell structure which can result in its ability to attach, penetrate and fertilize the egg. These two criteria can prove to be major causes in failure to achieve pregnancy after injection to the uterus. Therefor the leading principal in a successful IUI is a meticulous painstaking sorting out of the sperm cells regarding those two criteria. In most private labs (and even some hospitals) the preparation of sperm for injection is done via a method called "sperm washing" in which the sperm cells as well as anything else in the semen (faulty sperms, inflammation cell etc.) is separated from the semen fluid and injected to uterus. While this method somewhat helps sperm cells with low movability it has zero effect on sperm with abnormal structure. An alternative to sperm washing is "sperm improvement" which is performed either via SU (swim up) or DGC methods. In the SU sperms with optimal movability are selected (this doesn’t mean that they have a normal structure). On the other hand, in the DGC (which yield better results) the more mature and better structured sperm cells with a better movability are picked and thus the chances for achieving pregnancy dramatically increase. Gale-Onn labs exclusively use the DGC method as part of IUI sperm preparation.
Double Gradient Technic

Further improvement of sperm sorting out and increase of prospects for pregnancy via IUI, is achieved by adding an additional gradient technic, developed in the Gale-Onn labs, called the "Double Gradient" via the Double Gradient the sperm cells are separated with regards to both criteria; movability and structure. Preparation of the sperm cells using the Double Gradient technic results in significant increase in movability (more than 90% increase) and percentage of sperm cells with normal structure (more than 200% increase) when compared to situation before applying the Double Gradient technic. Our experience shows that in many cases repeated failures in IUI in spite of DGC method applying the Double Gradient technic can achieve the desired pregnancy and save the couple going through the unpleasant and costly IVF treatment.

B. Thoroughly analyzing past failure of IUI or IVF (In Vitro Fertilization) treatment
Continuing treatments after repeated failure in IUI or IVF requires immense mental resources and often leads to despair. Often times couples and doctors who tried everything modern medicine has to offer feel helpless and frustrated after yet another failure. While there are many complex reason and factors affecting fertility and achievement of pregnancy and it is not always easy to find a solution, it is imperative to thoroughly deal with all the involved factors. Therefore, we at the Gale-Onn apply four tests that can help your physician to select the right approach and treatment:

• 1. DNA Fragmentation Test (using the Halosperm method) – the hereditary material tightly condensed in the sperm's head and the minerals and unique proteins have a vital role in maintaining its normalcy. In part of the sperm cells the hereditary material is damaged and fragmentation in the DNA molecule (in which the genetic traits are encrypted). Increase in DNA's fragmentation can explain failed embryos development in IVF, repeated failure in achieving pregnancy or lack of progress towards a viable embryo. Using the Halosperm method we can identify and measure the DNA fragmentation and see if that is the cause for the problems and use the proper technology to solve it.
• 2. Test Using F.I.S.H. (Fluorescent in Situ Hybridization) technology. This test indicates if the chromosomes have been properly distributed. As part of the test several chromosomes are tested in order to see if a single representative of each is present in the sperm cell.
• 3. Acrosome Test - is an organelle that develops over the anterior half of the head in the sperm cell. Enzymes in the acrosome play major role in the sperm penetrating the egg and defects in the acrosome damage the sperm's ability to complete the fertilization. The acrosome florescent test is accurate and allows us to asses it ability to fertilizes the egg.
• 4. PLCζ protein test [not yet available] – the PLCζ plays a critical role in egg activation and the start of the egg's fertilization and division to cells. The test is primarily dedicated to couples who following ICSI (insertion of sperm cells to eggs via micromanipulation) as part of IVF treatment have not seen fertilized eggs or that the fertilized eggs percentage had been very low. The PLCζ test allows us to see if the lack of PLCζ in the sperm cells is the cause.

C. Thoroughly analyzing cause for apparent lack of sperm cells seen in standard sperm test (Azoospermia).

Lack of sperm cells in the semen fluid can indicate of either sperm number to low to be detected via a standard sperm test or total lack of production of sperm cell due to various diseases. Both of the above are categorized under NOA: Non-Obstructive Azoospermia. Another possibility is that the lack of sperm in the seamen is due to blockage in the sperm's path (OA: Obstructive Azoospermia) this can be accompanied with complete or severe lack of semen fluid (Aspermia or Sever Hypocupremia). Aspermia or Sever Hypocupremia increase the existence of Retrograde Ejaculation, meaning that most/all of semen fluid reaches the bladder and doesn't exit through the penis. In order to fully and accurately diagnose the causes for the lack of sperm (and thereby provide the proper treatment) the following test are performed by us:

• 1. Intensive Sperm Test – this include a multilevel test algorithm which allows to locate as low as 10 sperm cells using: Upright and Inverted (DIC optic) microscopes.
• 2. Locating fructose in the seamen fluid (this is done if no sperm cells have been found in the intensive sperm test) – lack of fructose can indicate blockage.
• 3. Round Cells' FISH Test. Confirmation of the existence of round cells increases by up to 50% the prospects of extracting sperm cells using TESE.

Presents of Sperm Cells in the urine. This provides indication of Retrograde Ejaculation which is treatable by extracting using highly sophisticated technic to extract sperm from the urine